Publications: Department of Genetic Engineering and Biotechnology

Home/GEB/Publications
Journal Publications
  1. Zaman, N., Seitz, K., Kabir, M. M., George-Schreder, L., Shepstone, I., Liu, Y., Zhang, S., and Krysan, P. J. A Forster resonance energy transfer sensor for live-cell imaging of mitogen-activated protein kinase activity in Arabidopsis. The Plant Journal, 97:970-983, 2019.

  2. Kabir, M. M., Hossain, C. F., and Shimizu, K. Can bio-plastics replace non-biodegradable plastics? J. Appl. Biotechnol. Bioeng, 3(4):00074, 2017.

  3. Hossain, C. F., Al-Amin, M., Sayem, A. S. M., Siragee, I. H., Tunan, A. M., Hassan, F., Kabir, M. M., and Sultana, G. N. N. Antinociceptive principle from Curcuma aeruginosa. BMCComplementary & Alternative Medicine, 15(191):1-7, 2015.

  4. Crochet, A. P., Kabir, M. M, Francis, M. B., and Paavola, C. D. Site-selective dual modification of periplasmic binding proteins for sensing applications. Biosensors & Bioelectronics, 26:55-61, 2010.

  5. Sporty, J. L., Kabir, M. M., Turteltaub, K. W., Ognibene, T., Lin, S-J., and Bench, G. Single sample extraction protocol for the quantification of NAD and NADH redox states in Saccharomyces cerevisiae. J. Sep. Sci., 31:3202-3211, 2008.

  6. Vogel, J. S., Palmblad, N. M., Ognibene, T., Kabir, M. M., Buchholz, B. A., and Bench, G. Biochemical paths in humans and cells: Frontiers of AMS bioanalysis. Nucl. Instr. Methods Phys. Res. B, 259:745-751, 2007.

  7. Kabir, M. M., and Shimizu, K. Investigation into the effect of soxR and soxS genes deletion on the central metabolism of Escherichia coli based on gene expressions and enzyme activities. Biochem. Eng. J., 30:39-47, 2006.

  8. Kabir, M. M., Ho, P. Y., and Shimizu, K. Effect of ldhA gene deletion on the metabolism of Escherichia coli based on gene expression, enzyme activities, intracellular metabolite concentrations, and metabolic flux distribution. Biochem. Eng. J., 26:1-11, 2005.

  9. Kabir, M. M., and Shimizu, K. Metabolic regulation analysis of icd-gene knockout Escherichia coli based on 2D electrophoresis with TOF-MS and enzyme activity measurements. Appl. Microbiol. Biotechnol., 65:84-96, 2004.

  10. Kabir, M. M., and Shimizu, K. Fermentation characteristics and protein expression patterns in a recombinant Escherichia coli mutant lacking phosphoglucose isomerase for poly (3-hydroxybutyrate) production. Appl. Microbiol. Biotechnol., 62:244-255, 2003.

  11. Kabir, M. M., and Shimizu, K. Gene expression patterns for metabolic pathway in pgi knockout Escherichia coli with and without phb genes based on RT-PCR. J. Biotechnol., 105:11-31, 2003.

  12. Kabir, M. M., and Shimizu, K. Proteome analysis of a temperature-inducible recombinant E. coli for poly-beta- hydroxybutyrate production. J. Biosci. Bioeng., 92:277-284, 2001.

        

Publications of Dr. Mohiuddin Kabir in ResearchGate

Conferences & Seminars
  1. Islam, S., Kabir, M., Hasanuzzaman, M., Sajib, M. S. I and Saha, S. K. Identification and differentiation of Acinetobacter calcoaceticusAcinetobacter baumannii complex: Common nosocomial infections among children. 1st Multidisciplinary International Conference on the Social and Life Sciences: Gender, Health, Information, and the Environment (ICSL 2020), February 27-29, 2020. East West University, Aftabnagar, Dhaka, Bangladesh.

  2. Kabir, R., Islam, M. and Kabir, M. Future perspective of biodegradable plastic. 1st Multidisciplinary International Conference on the Social and Life Sciences: Gender, Health, Information, and the Environment (ICSL 2020), February 27-29, 2020. East West University, Aftabnagar, Dhaka, Bangladesh.

  3. Krysan, P., Seitz, K., Zaman, N. and Kabir, M. Fluorescent sensors for imaging MAP kinase activity in Arabidopsis. 43rd FEBS Congress: Biochemistry Forever, July 7-12, 2018. Prague, Czech Republic.

  4. Paavola, C., Kabir, M. M., Crochet, A., and Francis, M. Protein-based sensors to detect chemical markers of life. Astrobiology Science Conference (AbSciCon), April 14-17, 2008. Santa Clara Convention Center, Santa Clara, California, USA.

  5. Kabir, M. M., Ognibene, T., Palmblad, M., Ubick, E. A., Lin, S-J., Vogel, J. S. and Bench, G. Metabolomics from single cells via AMS. 10th International Meeting of the Association of Biomolecular Resource Facilities (ABRF), February 11-14, 2006. Long Beach Convention Center, Long Beach, California, USA.

  6. Kabir, M. M., Ognibene, T., Palmblad, M., Ubick, E. A., Lin, S-J., Vogel, J. S. and Bench, G. Measurement of NAD and NADH from a single cell via AMS. Annual Poster Presentation, February 14, 2006. Energy and Environmental Directorate, Lawrence Livermore National Laboratory, Livermore, California, USA.

  7. Kabir, M. M., Ognibene, T., Ubick, E. A., Palmblad, M., Lin, S-J., Bench, G., and Vogel, J. S. Methods for single cell AMS. 10th International Conference on Accelerator Mass Spectrometry (AMS-10), September 5-10, 2005. University of California, Berkeley, California, USA.

  8. Bench, G., Buchholz, B. A., Hillegonds, D., Kabir,M. M., Ognibene, T., Palmblad, M., and Vogel, J. S. (2005). Frontiers of AMS in bioscience from cells to humans. Workshop on “Accelerator Mass Spectrometry in Low Dose Bioscience”, September 9, 2005. University of California, Berkeley, California, USA.

  9. Kabir, M. M., and Shimizu, K. Metabolic regulation analysis of E. coli with ldhA gene knockout based on enzyme activities and gene expressions. Annual Meeting of the Society for Biotechnol., September 21-23, 2004. Meijyo University (Tenpaku Campus), Nagoya, Japan.

  10. Kabir, M. M., and Shimizu, K. The effect of icdA gene knockout on the metabolism of E. coli. Regional Meeting of the Society for Biotechnology, December 13, 2003. Minami Kyushu Daigaku, Miyazaki, Japan.

  11. Kabir, M. M., and Shimizu, K. Metabolic pathway analysis of pgi knockout Escherichia coli for PHB production based on RT-PCR. Annual Meeting of the Society for Biotechnology, September 16-18, 2003. Kumamoto University (Kurokami Campus), Kumamoto, Japan.

  12. Kabir, M. M., and Shimizu, K. Metabolic flux analysis of Escherichia coli based on 13C-labeling experiments and metabolic regulation analysis based on gene and protein expressions. International Conference on Emerging Frontiers at the Interface of Chemistry and Biology (ICB-2003), April 28-30, 2003. Trivandrum, Kerala, India.

  13. Kabir, M. M., and Shimizu, K. Genetic and fermentation studies on the production of poly(3-hydroxybutyrate) by recombinant phosphoglucose isomerase knock-out Escherichia coli. Regional Meeting of the Society for Bioscience and Bioengineering, December 14, 2002. Ryukyu University, Okinawa, Japan.

  14. Kabir, M. M., and Shimizu, K. Proteome analysis of recombinant pgi knock-out E. coli for PHB production. 80th Anniversary of the Foundation and Annual Meeting of the Society for Bioscience and Bioengineering, October 28-30, 2002. Grand Cube Osaka (Osaka International Convention Centre), Nakanoshima, Kita-ku, Osaka, Japan.

  15. Kabir, M. M., and Shimizu, K. Effect of culture condition on protein expressions of a recombinant E. coli. 67th SCEJ Annual Meeting of the Society of Chemical Engineers Japan, March 27-29, 2002. Fukuoka Institute of Technology, Fukuoka, Japan.

  16. Kabir, M. M., and Shimizu, K. Proteome analysis together with metabolic flux distribution of a recombinant E. coli having phb genes. Regional Meeting of the Society for Bioscience and Bioengineering, December 8, 2001. Kyushu University, Fukuoka, Japan.

  17. Kabir, M. M., and Shimizu, K. Proteome analysis of recombinant E. coli with metabolic flux analysis for PHB production. Annual Meeting of the Society for Bioscience and Bioengineering, September 26-28, 2001. Yamanashi University, Kofu, Yamanashi, Japan.

  18. Kabir, M. M., and Shimizu, K. Proteome analysis of a temperature-induced recombinant E. coli having phb genes for PHB production. SCEJ Regional Meeting, July 27, 2001. Senkyo Building (8th floor), Fukui, Japan.

  19. Kabir, M. M., and Shimizu, K. Proteome analysis of a recombinant E. coli  having phb genes for PHB production. 66th SCEJ Annual Meeting of the Society of Chemical Engineers Japan, April 2-4, 2001. Hiroshima University, Hiroshima, Japan.

  20. Kabir, M. M., and Shimizu, K. Proteome analysis of a temperature-inducible recombinant E. coli for PHB production. Regional Meeting of the Society for Bioscience and Bioengineering, December 9, 2000. Kyushu Institute of Technology, Iizuka, Fukuoka, Japan.

  21. Shimizu, K., and Kabir, M. M. Analysis of microbial metabolic networks in consideration of the revelation of genetic control. Conference on the Genome Information Science, September 22-23, 2000. Hotel Associa Shizuoka Terminal, Shizuoka, Japan.

  22. Shi, H., Kyuwa, K., Takasu, M., Kabir, M. M., and Shimizu, K. Temperature-induced expression of phb genes in Escherichia coli and effect of temperature patterns on the production of PHB. Annual Meeting of the Society for Bioscience and Bioengineering, August 3-5, 2000.  Hokkaido University, Sapporo, Hokkaido, Japan.

Journal Publication
  1. Marzia Sultana, Suraia Nusrin, Nur A. Hasan, Abdus Sadique, Kabir U. Ahmed, Atiqul Islam, Anwar Hossain, Ira Longini, Azhar Nizam, Anwar Huq, Abul K. Siddique, David A. Sack, Richard B. Sack, Rita R. Colwell, Munirul Alam. Biofilms comprise a component of the annual cycle of Vibrio cholerae in the Bay of Bengal Estuary. mBIO®, 2018, 9 (2), 1-13.
  2. Richard Man Kit Yu, Gayathri Chaturvedi, Kwan Hok Tong, Suraia Nusrin, John P Giesy, Rudolf S Wu and Richard Y C Kong. Evidence for Epigenetic Regulation of Steroidogenic Gene Expression by Hypoxia. Environmental Science and Technology, 2015, 49(2), 1138-1147.
  3. Suraia Nusrin, Kwan Hok Tong, Gayathri Chaturvedi, Rudolf S Wu, John P Giesy and Richard Y C Kong. Regulation of CYP11B1 and CYP11B2 steroidogenic genes by hypoxia-inducible miR-10b in H295R cells. Marine Pollution Bulletin, 2014, 85, 344–351.
  4. N. A. Bhuiyan, Suraia Nusrin, M. Ansaruzzaman, A. Islam, M. Sultana, , M. Alam, M.A. Islam, A. Cravioto, A. K. Mukhopadhyay, G. B. Nair, J. C.L.Mwasna and H. P. Endtz. Genetic characterization of Vibrio cholerae O1 strains isolated in Zambia during 1996–2004 possessing the unique VSP-II region of El Tor variant. Epidemiology and Infection, 2011, 31, 1-9.
  5. Munirul Alam, Suraia Nusrin, Atiqul Islam, Nurul A. Bhuiyan, Niaz Rahim, Gabriela Delgado, Rosario Morales, Jose Luis Mendez, Armando Navarro, Ana I. Gil, Haruo Watanabe, Masatomo Morita, G. Balakrish Nair, and Alejandro Cravioto. Cholera between 1991 and 1997 in Mexico Was Associated with Infection by Classical, El Tor, and El Tor Variants of Vibrio choleraeJournal of Clinical Microbiology, 2010, 48, 3666–3674.
  6. Nurul Amin Bhuiyan, Suraia Nusrin, Munirul Alam, Haruo Watanabe, Masatomo Morita, Alejandro Cravioto, T. Ramamurthy, and G. Balakrish Nair. Changing Genotypes of Cholera Toxin (CT) of Vibrio cholerae O139 in Bangladesh and Description of Three New CT Genotypes. FEMS Immunology & Medical Microbiology, 2009, 57, 136-141.
  7. Suraia Nusrin, Ana I. Gil, N. A. Bhuiyan, Ashrafus Safa, Masahiro Asakura, C. F. Lanata, E.R. Hall, H. Miranda, B. Huapaya, C. Vargas G, M.A. Luna, D.A.Sack, Shinji Yamasaki and G. Balakrish Nair. The Peruvian El Tor strains of Vibrio cholerae O1 have a distinct region in the Vibrio seventh pandemic island-II that differentiates them from the seventh pandemic strains of other continents. Journal of Medical Microbiology, 2009, 58, 342-354.
  8. Ashrafus Safa, Nurul A. Bhuiyan, Denise Murphy, John Bates, Suraia Nusrin, Richard Y. C. Kong, Manas Chongsanguan, Wanpen Chaicumpa and G. Balakrish Nair. Multilocus genetic analysis reveals that the Australian strains of Vibrio choleraeO1 are similar to the pre-seventh pandemic strains of the El Tor biotype. Journal of Medical Microbiology, 2009, 58, 105-111.
  9. Masatomo Morita, Makoto Ohnishi, Eiji Arakawa, N.A. Bhuiyan, Suraia Nusrin, Munirul Alam, A.K. Siddique, Firdausi Qadri, Hidemasa Izumiya, G. Balakrish Nair and Haruo Watanabe. Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor. Microbiology and Immunology, 2008, 52, 314-317.
  10. Ana I. Gil, Hernan Miranda, Claudio F. Lanata, Ana Prada, Eric R. Hall, Carmen M. Barreno, Suraia Nusrin, Nurul A. Bhuiyan, David A. Sack, Gopinath Balakrish Nair. O3:K6 Serotype of Vibrio parahaemolyticus identical to the global pandemic clone associated with diarrhea in Peru. International Journal of Infectious Diseases, 2007, 11, 324-328.
  11. Ashrafus Safa, N. A. Bhuyian, Suraia Nusrin, M. Ansaruzzaman, Munirul Alam, T. Hamabata, Yoshifumi Takeda, David A. Sack, and G. Balakrish Nair. Genetic Characteristics of Matlab variants of Vibrio cholerae O1 that are hybrids between Classical and El Tor biotypes. Journal of Medical Microbiology, 2006, 55,1563-1569.
  12. G. Balakrish Nair, Ashrafus Safa, N.A. Bhuiyan, Suraia Nusrin, Denise Murphy, Carolyn Nicol, Mary Valcanis, Steven Iddings, Ili Kubuabola, and Hassan Vally. Isolation of Vibrio cholerae O1 similar to pre-seventh pandemic El Tor strains during an outbreak of gastrointestinal disease in an island resort in Fiji. Journal of Medical Microbiology, 2006, 55, 1559-1562.
  13. Munirul Alam, Nur-A-Hasan, A. Sadique, N. A. Bhuiyan, Kabir U. Ahmed, Suraia Nusrin, G. Balakrish Nair, K. Siddique, R. Bradly Sack, David A. Sack, Anwar Huq, and Rita R. Colwell. 2006. Seasonal Cholera Caused by Vibrio cholerae Serogroups O1 and O139 in the Coastal Aquatic Environment of Bangladesh. Applied and Environmental Microbiology,June 2006, p. 4096-4104.
  14. Suraia Nusrin, G. Yeahia Khan, N. A. Bhuiyan, M. Ansaruzzaman, M. A. Hossain, Ashrafus Safa, Rasel Khan, Shah M. Faruque, David A. Sack, T. Hamabata, Yoshifumi Takeda, and G. Balakrish Nair. Diverse CTX Phages among Toxigenic Vibrio cholerae O1 and O139 strains isolated between 1994 and 2002 in an area where Cholera is endemic in Bangladesh. Journal of Clinical Microbiology. 2004; 42:5854-5856.
Conference & Seminars
  1. Suraia Nusrin, Kwan Hok Tong, Gayathri Chaturvedi, Rudolf S Wu, John P Giesy and Richard Y C Kong. Regulation of CYP11B1 and CYP11B2 steroidogenic genes by hypoxia-inducible miR-10b in H295R cells. 7th International Conference on Marine Pollution and Ecotoxicology, 17-21 June, 2013, Hong Kong.
  2. Suraia Nusrin, Rudolf S Wu, John P Giesy and Richard Y C Kong. Effect of Hypoxia–inducible Factors (HIFs) on microRNA expression and steroidogenesis in the H295R human adrenocortical cell line. Centre for Marine Environmental Research & Innovative Technology (MERIT) Annual Symposium, 5th January – 6th January 2012.
  3. G. Chaturvedi , Suraia Nusrin,  R.S.S.Wu, J.Giesy, and Richard.Y.C. Kong. Effects of Hypoxia–inducible Factors on steroidogenesis: Identification of Molecular Biomarkers of Endocrine Disruption. 15th International Symposium on Toxicity Assessment, 3-8 July 2011, Hong Kong.
  4. Suraia Nusrin, G. Chaturvedi and Richard Y. C. Kong, R. S. S. Wu and J. Giesy. Effect of Hypoxia–inducible Factors (HIFs) on steroidogenic genes of the stress axis in H295R cells. 5th PhD student Workshop, 18-19 April 2011, Suzhou, China.
  5. G. Chaturvedi , Suraia Nusrin,  R.S.S.Wu, J.Giesy, and Richard.Y.C. Kong. Effect of Hypoxia–inducible Factors (HIFs) on microRNA expression and steroidogenesis in the H295R human adrenocortical cell line. Centre for Marine Environmental Research & Innovative Technology (MERIT) Annual Symposium, 6th January – 7th January 2011.
  6. Munirul Alam, A.K. Siddique, G. Balakrish Nair, R.B. Sack, David A. Sack, Nur A. Hasan, Atiqul Islam, A. Sadique, M. Sultana, K.U. Ahmed, W.B. Chowdhury, N.A. Bhuiyan, S.U. Ahmed, A. Iqbal, Suraia Nusrin, Niaz Rahim,C.J. Grim, Hubert P. Endtz, Alejandro Cravioto, Anwar Huq, and Rita R. Colwell. Vibrio cholerae Serogroup O1 and O139 Causing Endemic Cholera in the Coastal Aquatic Environment of Bangladesh: A Molecular Epidemiological, Ecological and Phylogenetic Study. 12th Annual Scientific Conference (ASCON), 9-12 February 2009, Dhaka, Bangladesh.
  7. N.A. Bhuiyan, Suraia Nusrin, Munirul Alam, A.K. Siddique, Haruo Watanabe, and G. Balakrish Nair. Appearance of Three New Genotypes of Cholera Toxin B- subunit in Vibrio cholerae O139 Isolated in Bangladesh. 12th Annual Scientific Conference (ASCON), 9-12 February 2009, Dhaka, Bangladesh.
  8. Munirul Alam, G.Balakrish Nair, A.K. Siddique, R. Bradley Sack, David A. Sack, Nur- A-Hassan, Marzia Sultana, Abdus Sadique, N.A Bhuiyan, Kabir U. Ahmed, Suraia Nusrin, Atiqul Islam, Wasimu B. Cowdhury, Christopher J. Grim, Anwarul Huq, and Rita R. Colwell. Vibrio Cholerae serogroups O1 El Tor and O139 Bengal native to the aquatic environment of the coastal villages of the Bay of Bengal are the source of endemic cholera: A follow-up. 42nd US-Japan Cooperative Medical Program on Cholera and other Enteric Diseases. 5-7, Dec 2007, Austin, Texas, USA.
  9. Suraia Nusrin, Ana I. Gil, N. A. Bhuiyan, Ashrafus Safa, Masahiro Asakura, C. F. Lanata, E. Hall, H. Miranda, B. Huapaya, C. Vargas G, M.A. Luna , D.A. Sack, Shinji Yamasaki and G. Balakrish Nair. The Peruvian El Tor strains of Vibrio cholerae O1 have a distinct region in the Vibrio seventh pandemic island-II. 42nd US-Japan Cooperative Medical Program on Cholera and other Enteric Diseases. 5-7, Dec 2007, Austin, Texas, USA.
  10. G. Balakrish Nair, N.A. Bhuiyan, Suraia Nusrin, Munirul Alam, A. Safa, Firdausi Qadri, S. M. Faruque, A. K. Siddique, Yoshifumi Takeda and David A. Sack. Molecular basis of the emergence of a new more severe form of cholera. Vibrio 2007, the second conference on the Biology of Vibrios, 28th Nov –1st Dec 2007, Institut Pasteur, Paris.
  11. Munirul Alam, Nur- A-Hassan N.A Bhuiyan A.K. Siddique, R. Bradley Sack, David A. Sack, Marzia Sultana, Abdus Sadique, Kabir U. Ahmed, Suraia Nusrin, Atiqul Islam, Wasimu B. Cowdhury, Anwarul Huq, Rita R. Colwell and G.Balakrish Nair. Antibiotic Resistance Profiles of Vibrio cholerae O1 and O139 Isolated from Coastal Ecosystim of Bangladesh. 11th Annual Scientific Conference (ASCON), 4-6 March 2007, Dhaka, Bangladesh.
  12. Munirul Alam, A.K. Siddique, R. Bradley Sack, David A. Sack, Nur- A-Hassan, N.A. Bhuiyan, Marzia Sultana, Abdus Sadique, Kabir U. Ahmed, Suraia Nusrin, Atiqul Islam, Wasimu B. Cowdhury, G.Balakrish Nair,Anwarul Huq and Rita R. Colwell. Ecology and Molecular Traits of Vibrio cholerae Serogroup O1 and O139 Causing. Cholera in Coastal Villages of Bay of Bengal. 11thAnnual Scientific Conference (ASCON), 4-6 March 2007, Dhaka, Bangladesh.
  13. Munirul Alam, G.Balakrish Nair, A.K. Siddique, R. Bradley Sack, David A. Sack, Nur- A-Hassan, Marzia Sultana, Abdus Sadique, N.A Bhuiyan, Kabir U. Ahmed, Suraia Nusrin, Atiqul Islam, Wasimu B. Cowdhury, Anwarul Huq, and Rita R. Colwell. Vibrio Cholerae serogroups O139 Bengal Competes with Serogroup O1 for Endemicity in the Coastal aquatic environment of Bangladesh. 41st US-Japan Cooperative Medical Program on Cholera and other Enteric Diseases. 5-7, Nov 2006, Gifu, Japan.
  14. Munirul Alam, Nur-A-Hasan, A. Sadique, N. A. Bhuiyan, Kabir U. Ahmed, Suraia Nusrin,G. Balakrish Nair, K. Siddique, R. Bradly Sack, David A. Sack, Anwar Huq, and Rita R. Colwell. Seasonal Cholera Caused by Vibrio cholerae Serogroups O1 and O139 in the Coastal Aquatic Environment of Bangladesh. 11th Asian Conference on Diarrhoeal Diseases and Nutrition, March 8-10, 2006, The Rama Gardens Hotel, Bangkok, Thailand.
  15. Ashsrafus Safa, N. A. Bhuiyan, Suraia Nusrin, Munirul Alam, T. Hamabata, Y. Takeda, David A. Sack and G. Balakrish Nair. Matlab variants of Vibrio cholerae possesses gene clusters specific to pandemic strains.8th Commonwealth Congress on Diarrhoea and Malnutrition, 6-8 February 2006, ICDDR,B, Dhaka, Bangladesh.
  16. G. Balakrish Nair, N. A. Bhuiyan, Suraia Nusrin, A. Safa, M. Ansaruzzaman, S. M. Faruque, T. Hamabata, Yoshifumi Takeda and David A. Sack. The Matlab Variants of Vibrio cholerae O1: Do they deserve a new biotype status? Fortieth Anniversary of United States- Japan Cooperative Medical science Program, December 7-10, 2004, Kyoto, Japan.
Expertise
  • Different cell cultures for example H295R human adrenocortical cell line, 293FT cell line, HT1080 human fibrosarcoma CCL-12 cell line, and HeLa CCL-2 cell line.
  • Molecular biological techniques such as construction of plasmids overexpressing the desired genes using the GATEWAY™ Cloning Technology (Invitrogen, USA), gene knockdown by RNA interference, gene sequencing, sequencing data analysis (Artemis, Chromas, BioEdit, ClustalX, GeneDoc, MEGA etc), site-directed mutagenesis, quantitative real time PCR, electroporation of miRNA (Neon, Invitrogen), Luciferase reporter assay, SDSPAGE and Western blotting and most importantly transduction of H295R cells with lentivirus.
  • Involved with several important projects at ICDDR,B specifically isolation of Vibrio Cholerae from clinical samples with CDC and the epidemiology and ecology of V. Cholerae in the Southern part of Bangladesh with NIH.
Analysis of Different Prognostic Indicators for Malnutrition and Shigella flexneri Infection Among the Children in Bangladesh

The present study investigates into some socio-economic, demographic, and nutritional features that can predispose Bangladeshi children to malnutrition and Shigella flexneri infection. Significant prognostic indicators associated with malnutrition were mother’s illiteracy (unadjusted odds ratio, OR = 2.580; 95% confidence interval, CI = 1.134–5.867 and adjusted odds ratio, ORa, 3.814; 95% CI = 1.124–12.943), low birth weight (OR = 3.143; 95% CI = 1.2–8.232 and ORa = 2.404; 95% CI = 0.870–6.644) and poor socioeconomic status (OR = 2.549; 95% CI = 1.382–4.701 and ORa = 1.808; 95% CI = 0.852–3.838). Age was the strongest predictor for the acquisition of S. flexneri infection in the studied population (OR = 5.472; 95% CI = 2.583–11.592 and ORa = 5.808; 95% CI = 2.420–13.942). The severity of malnutrition was significantly (P = 0.033) related to seroprevalence of S. flexneri infection. To reduce malnutrition emphasis should be given on controlling the incidence of low birth weight, improving the literacy status especially of mothers and narrowing the gap between different socio-economic levels. Malnourished children should be examined for severity and direct intervention therapy should be given when necessary.

Drug resistance and curli fimbrination of Escherichia coli isolated from Bangladeshi patients with urinary tract infections

A total of 27 isolates from patients with urinary tract infection (UTI) were screened and of these 12 were found to be Escherichia coli. All the E. coli isolates were multidrug resistant. Among the antibiotics, imipenem and polymyxin B were found to be the best, while oxacillin, cefsulodine and methicillin proved to be worst in effectiveness against the studied E. coli isolates. Only 42% of the E. coli were susceptible to trimethoprim-sulfamethoxazole (TMP–SMX), the current drug of choice for treating UTI. Middle ranged plasmids were observed in the studied isolates. Five strains expressed curli fimbriae and two elaborated cellulose; two other strains produced both curli and cellulose as extracellular matrix component.

Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8

Background

R2 elements are a clade of early branching Long Interspersed Elements (LINEs). LINEs are retrotransposable elements whose replication can have profound effects on the genomes in which they reside. No crystal or EM structures exist for the reverse transcriptase (RT) and linker regions of LINEs.

Results

Using limited proteolysis as a probe for globular domain structure, we show that the protein encoded by the Bombyx mori R2 element has two major globular domains: (1) a small globular domain consisting of the N-terminal zinc finger and Myb motifs, and (2) a large globular domain consisting of the RT, linker, and type II restriction-like endonuclease (RLE). Further digestion of the large globular domain occurred within the RT. Mapping these RT cleavages onto an updated model of the R2Bm RT indicated that the thumb of the RT was largely protected from proteolytic cleavage. The crystal structure of the large globular domain of Prp8, a eukaryotic splicing factor, was a major template used in building the R2Bm RT model, particularly the thumb region. The large fragment of Prp8 consists not only of a RT similar to R2Bm, but also an RLE and a linker connecting the two regions. The linker sequences adjacent to the RLE in LINEs and Prp8 share a set of two important α-helices and a (presumptive) knuckle/ββα structural motif that are closely associated with the thumb. The RLEs of LINEs and Prp8 share a unique catalytic core residue spacing as well as other key residues.

Conclusions

The protein encoded by RLE LINEs consists of two major globular domains. The larger of the two globular domain contains the RT, linker, and RLE and is similar to the large fragment of the spliceosomal protein Prp8. The similarities are suggestive of possible common ancestry.

Influence of some sociodemographic factors on Shigella flexneri infection and malnourishment among the children population in Bangladesh
Journal Publications

  • Kawsar AhmedAbdullah-Al-Emran, TasnubaJesmin, Roushney Fatima Mukti, Zamilur Rahman, Farzana Ahmed. Early Detection of Lung Cancer Risk Using Data Mining. Asian Pacific Journal of Cancer Prevention, Vol 14 (1), p595-598, 2013.
  • Roushney Fatima MuktiPratul Dipta Somadder, Abdullah Al Emran, Farzana Ahmed, Iqbal Bin Imran, Ananya Malaker, Sabina Yeasmin. Score Based Risk Assessment of Lung Cancer and Its Evaluation for Bangladeshi People. Asian Pacific Journal of Cancer Prevention, Vol 15 (17), p7021-7027, 2014.
  • Roushney Fatima MuktiMd. Miraj Kobad Chowdhury, Md. Ausrafuggaman Nahid, Md. Mizanur Rahaman, M Aftab Uddin. Antibacterial Activity and Physicochemical Properties of Florally Diversified Bangladeshi Honeys. Bangladesh J Microbiol, Volume 33, Number 1&2, June-December 2016, pp 05-09.
  • Roushney Fatima MuktiSanjida Sakhawat Sinthee. Metagenomic Approach: Transforming In Silico Research for Improved Biogas Production. International Journal of Applied Sciences and Biotechnology, Vol-7, issue-1, 2019. 
  • Roushney Fatima MuktiShuborno Islam. Epigenetic contributions on mental health, neurodevelopmental and neurodegenerative disorders. International Journal of Biosciences, Vol. 14, No. 1, p. 38-52, 2019. 
Conference & Seminars

Roushney Fatima Mukti. Reproductive and Socio-demographic Factors Associated with Ovarian Cancer Risk among Bangladeshi Women. 5th International Conference on Public Health 2019 (ICOPH 2019), Kuala Lumpur, Malaysia, July 10-12, 2019. 

A. S. M. Mahadiuzzman, Md. Arafat Rashid, Rozely Hussain, RejaulKhalique Miah, Roushney Fatima MuktiMohiuddin Kabir. Risk Factor Assessment of Coronary Artery Disease among Bangladeshi People. National Conference on Biochemistry and Molecular Biology for Life Sciences, University of Dhaka, Dhaka, Bangladesh, December 10, 2016.

Roushney Fatima Mukti, Md. Miraj Kobad Chowdhury, Md. Aftab Uddin. Fermentative Potency of Yeasts Isolated from Bangladeshi Honey. International Conference of Biotechnology in Health and Agriculture, organized by GNOBB, Dhaka, January 9-10, 2015.

Roushney Fatima Mukti, A.K.M. Mohiuddin. Epigenetics: promising research in the progression and treatment of Alzheimer Disease. Conference of the Bangladesh Society for Biochemistry and Molecular Biology (BSBMB), University of Chittagong, January 12-13, 2013.

Roushney Fatima Mukti, Abdullah-Al-Emran. Metagenomic approach: Transforming In silico research for improved biogas production. International Training Workshop on Bioinformatics and Computational Biology in South Asian Perspectives, University of Science & Technology Chittagong (USTC), Bangladesh, September 25-26, 2012.

Journal Publications

1.  AMAM Zonaed Siddiki, Sohana Akter Mina, Zinat Farzana, Bibi Ayesa, Rasel Das, Mohammad Alamgir Hossain. Molecular characterization of Cryptosporidium Xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene. Asian Pacific Journal of Tropical Biomedicine, 5(3):  202-207, 2015.

2.  Nazneen Naher Islam, Zinat Farzana, A. M. Masudul Azad Chowdhury, Adnan Mannan, K. M. Kamaruddin, A.M.A.M. Zonaed Siddiki and Inkeyas Uddin. Characterization of Bovine Subclinical Mastitis Caused by Staphylococcus aureus in Southern Bangladesh by Bacteriological and Molecular Approaches. Asian Journal of Biological Sciences, 7(1): 112, 2014.

3.  Nazneen Naher Islam, Suza Mohammad Nur, Zinat Farzana, Inkeyas Uddin, K. M. Kamaruddin and A.M.A.M. Zonaed Siddiki. Rapid Identification of Eosin Methylene Blue Positive E. coli by Specific PCR from Frozen Chicken Ringe in Southern Chittagong City of Bangladesh: Prevalence and Antibiotic Susceptibility. Microbiology Journal, 4(2): 27-40, 2014.



Conference & Seminars

1. Zinat Farzana, Nazneen Naher Islam, AMAM Zonaed Siddiki. “Molecular characterization of Staphylococcus sp. causing subclinical mastitis in dairy cows in Chittagong region”. 9th Annual Scientific Conference on ‘Food safety in Bangladesh: Issues and Challenges’, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong, Bangladesh, March 08-09, 2012.

2. Zinat Farzana, Nazneen Naher Islam, AMAM Zonaed Siddiki. “PCR based molecular diagnosis of dairy cattle’s subclinical mastitis caused by Staphylococcus sp.” Annual Conference of Bangladesh Society for Biochemistry and Molecular Biology (BSBMB), University of Chittagong, Bangladesh, January 12-13, 2013.

3. Zinat Farzana. Workshop on “Computational Biology, Research Method and Bioinformatics camp” organized by YoungBB (Young Biotechnologists of Bangladesh) held in Chittagong, Bangladesh, October 06-07, 2011.

4. Zinat Farzana. Training on “Genomics and Bioinformatics tools used in Computational Biology”. Directorate (Research and Extension), Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh, May 29-30, 2013.